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Scientific Software Services :: Bioinformatics :: Genetics :: Primer Premier 6

Primer Premier 6 #306

Primer Premier: A comprehensive primer design software

A Comprehensive PCR Primer Design Software

Primer Premier Features

Primer Design for Standard PCR Assays

Primer Premier is the most comprehensive software to design and analyze PCR primers.

Primer Premier's search algorithm finds optimal PCR, multiplex, SNP genotyping and degenerate primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in ranked order. Equipped with a handy calculator, you can easily manipulate sequences and analyze the results of your primer design.

Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.

Primer Design for SNP Genotyping Assays

With Primer Premier, you can load sequences from dbSNP and have the primers designed flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by specifying them as variation features in standard GenBank/dbSNP files. After specifying the SNPs, primers can be designed to amplify them for detection using a probe-based chemistry.

Multiplex Primer Design

For a multiplex experiment, Primer Premier enables you to design multiplex primers by launching a primer search in batch mode and then checking the cross reactivity of the primers designed. Primer Premier checks for all the possible reactivity and displays the most stable structure formed by each oligo. This functionality reduces false priming and ensures a strong signal strength.

Automatic Homology & Template Structure Avoidance

Primer Premier automatically interprets the BLAST search results and avoids those regions to design primers that have significant cross homologies with the database. These homologous regions are highlighted in the sequence view and are avoided during primer search.

Primer extension may be hindered due to the presence of template structures at extension temperature. To avoid all such regions, where the template may fold upon itself, the program utilizes a proprietary algorithm to check for possible secondary structures within the template at a folding temperature you specify. The regions involved in the formation of a secondary structure are underlined in the sequence view and are avoided while designing primers.

Avoiding homologous regions makes the oligos highly specific and avoiding template structures improves the efficiency of the designed primers.

and much more...

What's new in this version?

Primer Premier 6.00 Released
Primer Premier now comes with a new improved graphical user interface, making PCR primer design both intuitive and easy for users.

The primer search algorithm is improved considerably to avoid homologies and template structures, making the primers designed by Primer Premier highly specific and efficient. The primer specificity can be ascertained using verification BLAST.

Primer Premier can now help design 10-plex multiplex assay.

Pre-designed primers or published primers can now be analyzed. Primer Premier can even design a compatible primer given a forward or reverse primer.

With a sequence view now available, its easy to visualize the designed primers with respect to the sequence. You can copy-paste sequences from the view and analyze them in the program as well.

Primer Premier has enhanced sequence import limits. You can work with 5MB of sequence at a time and a total of 15MB of sequence can be loaded.

Support for latest operating systems for both Windows and Macintosh.

If you are interested to check out Primer Premier's functions by yourself, download a
Demo Version of Primer Premier here:

Demo for Windows

Demo for Mac

System Requirements

For Windows

Required

Recommended

CPU	Pentium IV 1.80 GHz	Pentium IV 3.00 GHz or higher
RAM	256 MB of available RAM	512 MB of available RAM
Hard Disk Drive Space	500 MB of available hard disk	1 GB of available hard disk
Screen Resolution	800 X 600	1024 X 768

Platforms supported for Windows: 98/2000/ME/XP/Vista/Windows 7

Power PC*

Required

Recommended

CPU	IMac Power PC G5 1.6 GHz	IMac Power PC G5 1.6 GHz
RAM	256 MB of available RAM	512 MB of available RAM
Hard Disk Drive Space	500 MB of available hard disk	1 GB of available hard disk
Screen Resolution	800 X 600	1024 X 768

* Tested on Mac OS 10.6

Intel®-based Mac*

Required

Recommended

CPU	Intel 1.8 GHz Intel Core Duo	Intel 1.8 GHz Intel Core Duo
RAM	256 MB of available RAM	512 MB of available RAM
Hard Disk Drive Space	500 MB of available hard disk	1 GB of available hard disk
Screen Resolution	800 X 600	1440 X 900

Pricing

Primer Premier Pricing & Packages

License Type	Commercial	Academic
Single User License	$ 1.185,00 NOW $ 1.125,75	Contact Us!
Competitive Upgrade	Contact Us!	Under Request

NOTE: If you want to purchase Academic License(s) or <3 Licenses with Volume Discount please contact us!

Details

Price: $1,125.75 (€ 1,013.18)

Options

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select System Type

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